Join us for a two part video blog series with one of the preeminent experts in the field of dermatologic imaging. Jon Holmes covers aspects of real-time, in-vivo imaging of MAP insertion behavior and measurements of relevant structural parameters governing MAP performance.
About Jon Holmes and Michelson Diagnostics Ltd. – Click Here.
Join us for a two part video blog series with one of the preeminent experts in the field of dermatologic imaging. Jon Holmes covers aspects of real-time, invivo imaging of MAP insertion behavior and measurements of relevant structural parameters governing MAP performance.
About Jon Holmes and Michelson Diagnostics Ltd. – Click Here.
Join us for a four part video blog series with one of the preeminent experts in the field. Professor Ryan Donnelly covers fundamentals and new aspects of Microneedle Array Patch translational research.
Click here for more about Professor Ryan Donnelly, PhD
Join us for a four part video blog series with one of the preeminent experts in the field. Professor Ryan Donnelly covers fundamentals and new aspects of Microneedle Array Patch translational research.
Click here for more about Professor Ryan Donnelly, PhD
Join us for a four part video blog series with one of the preeminent experts in the field. Professor Ryan Donnelly covers fundamentals and new aspects of Microneedle Array Patch translational research.
Click here for more about Professor Ryan Donnelly, PhD
Join us for a four part video blog series with one of the preeminent experts in the field. Professor Ryan Donnelly covers fundamentals and new aspects of Microneedle Array Patch translational research.
Click here for more about Professor Ryan Donnelly, PhD
Research efforts on MAPs are at an all-time high. It is important to understand skin reactions during and after MAP applications, and further gain insights on MAP changes themselves as well. Questions that may arise are:
Measuring geometric parameters such as microneedle depth of penetration, deformation, swelling and dissolution have been well described in the literature (Fig.1) [1-3]. We report on two aspects that describe changes in the skin and in more detail outline the dramatic difference of vascular expression upon MAP insertion and removal.
Using “super swelling” hydrogel-forming MAPs, basic insertion tests were carried out. VivoSight OCT imaging visualizes and quantifies areas of interest (Fig.2):
Visualizing the insertion behavior of MAPs and its structural impact on skin provides a good foundation to investigate the ensuing inflammatory reaction.
After MAP insertion, an immediate vascular response is set in motion. The measurable increase in vascular expression is a function of a multitude of parameters such as needle depth, needle spacing, subject predisposition, repeat applications etc…
Understanding these relationships is paramount, as they have a strong impact on the pharmacokinetic availability of active substances over time. Dynamic OCT is useful to analyze the evolving interdependencies, and we present here imaging and measurements of vascular changes due to MAP insertion:
Bottom: frame “side’ view of vertical skin slice with visible epidermis and red highlighted blood vessels.
Top right: en-face “top down” view of horizontal section at level of selectable depth (thin green line in bottom image). The beginning of the superficial vascular plexus comes into view.
Top left: 3D representation of vascular network at level of horizontal slice.
Global vascular metrics are automatically calculated to quantify the vascular network:
A hydrogel-forming MAP was applied to skin and held in place for 34 minutes with Tegaderm.
Vascular expression is more pronounced than without MAP and exhibits significantly different parameters:
(Horizontal cuts are taken at same depth for both cases 1 and 2)
Drug uptake is impacted by the skin vascular microcirculation and increased influx of immune cells from other parts of the body could possibly enhance an overall immune response to a vaccine deposited in the viable skin layer. Differences in vascular perfusion can be accurately measured in real-time and in-vivo. Quantification provides for objective comparison of vascular profiles and allows for more effective research on:
Investigating these and related topics will accelerate MAP development and market introduction.
References:
About Professor Ryan Donnelly, PhD:
https://pure.qub.ac.uk/en/persons/ryan-donnelly
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